Reverse Transcription-Quantitative Real-Time Polymerase Chain Reaction (RT-qPCR) for Gene Expression Analyses.

Albert Caballero-Solares,Jennifer R Hall,Xi Xue,Matthew L Rise

Methods in molecular biology (Clifton, N.J.)（2022）

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摘要

The reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR) is considered to be the gold standard for gene expression research. However, for this claim to be valid, RT-qPCR studies must test and optimize the quality of its RNA templates and assays. This chapter describes the experimental procedures required to generate reliable and reproducible gene expression results using RT-qPCR.

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关键词

Gene of interest,MIQE guidelines,Normalizer gene selection,Polymerase chain reaction,Primer pairs,Quality testing,RNA,Reverse transcription,cDNA

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