一株四环素耐药大肠杆菌耐药基因检测

LIU Xuesong,ZHANG Yan,WANG Shuang,YANG Xudong,ZHU Qinghe,CHEN Xi,XUE Zhanmei,WANG Hongbao,SHI Tongrui

Modern Animal Husbandry Science & Technology（2022）

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Abstract

黑龙江省齐齐哈尔市周边牛场发生了犊牛腹泻情况,通过检测发现致病菌为致病性大肠杆菌.通过纸片法药敏试验发现这株大肠杆菌对四环素极为耐药,通过PCR探究其携带的耐药基因,发现其携带tetK耐药基因.表明该株大肠杆菌通过tetK基因编码四环素类抗生素药物外排泵,将四环素类药物排出体外,保护细菌核糖体,从而使细菌产生耐药性.

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