Effect of Biostimulation on Response of Immature Teeth with Necrotic Pulp and Apical Periodontitis to Regenerative Endodontic Therapy in Immature Dogs Teeth

Purpose : This study was carried out to evaluate the effect of biostimulation on the regenerative response of immature teeth with necrotic pulp and apical periodontitis in dogs. Materials and Methods: Seventy-two root canals were employed in the present study as follows: Sixty root canals for experimental groups and twelve as positive and negative controls. After the induction of pulp necrosis and apical periodontitis, roots were divided into two groups, group I: Regenerative Endodontic Therapy (RET) with subsequent biostimulation (seven sessions at 808 nm diode laser at output power of 300 mW for 90 seconds) and group II: RET without biostimulation. The groups were followed up for 1, 2 and 3 months (subgroups A, B&C). The maturation of the roots was assessed both radiographically and histologically. All data were statistically analyzed. Results: the application of biostimulation in group I demonstrated marked increase in root length, thickness and decrease in apical diameter compared to group II however, it was statistically significant only in subgroup C (12.43% versus 7.66%, 33.09% versus 17.81% and 38.12% versus 23.35 % at third month) respectively (p<0.05). Furthermore, it showed histologically the highest score of vital tissue infiltration and least inflammatory scores which was statistically significant. Conclusion: Biostimulation enhanced the response of immature teeth with necrotic pulp and apical periodontitis to regenerative endodontic therapy improving root maturation. titled” Regenerative Endodontic Therapy in Immature Teeth with Necrotic Pulp and Apical Periodontitis using two Disinfection Protocols and Biostimulation”