The role of Lrp6-mediated Wnt/ß-catenin signaling in the cause and intervention of spinal neural tube defects in mice
Disease Models & Mechanisms(2022)
摘要
Neural tube defects (NTDs) are among common and severe birth defects with poorly understood etiology. Mutations in the Wnt coreceptor LRP6 are associated with NTDs in humans. Either gain-of-function (GOF) or loss-of-function (LOF) mutations of Lrp6 can cause NTDs in mice. NTDs in Lrp6-GOF mutants may be attributed to altered ß-catenin-independent noncanonical Wnt signaling. However, the mechanisms of NTDs in Lrp6-LOF mutants and the role of Lrp6-mediated canonical Wnt/ß-catenin signaling in neural tube closure remain unresolved. We previously demonstrated that ß-catenin signaling is required for posterior neuropore (PNP) closure. The current study shows that conditional ablation of Lrp6 in dorsal PNP causes spinal NTDs with diminished activities of Wnt/ß-catenin signaling and its downstream target gene Pax3 that is required for PNP closure. ß-catenin-GOF can rescue NTDs in Lrp6-LOF mutants. Moreover, maternal supplementation of a Wnt/ß-catenin signaling agonist can reduce the frequency and severity of spinal NTDs in Lrp6-LOF mutants by restoring Pax3 expression. Together, these results demonstrate the essential role of Lrp6-mediated Wnt/ß-catenin signaling in PNP closure, which may also provide a therapeutic target for NTD intervention through manipulation of canonical Wnt/ß-catenin signaling activities.
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