Preparation and Properties of Gold Nanocages/Hyaluronic Acid Core-shell Nanocarriers with pH/Enzyme/ Photothermal Multiple Responses

The core-shell nano drug loaded particle DOX@AuNC@HA???DAH???was prepared by a simple one potmethod using gold nanocage???AuNC???as the core???sulfhydryl modified hyaluronic acid???LC-HA???as the shell and doxo???rubicin hydrochloride???DOX???as the drug model.The gold nanocage provides a container for drug loading and endowsthe carrier with photothermal properties.The modified hyaluronic acid encapsulates the gold nanocage and providespH/enzyme response and targeted mediating functions.The structure of DAH was characterized???and the drugloading???controlled release performances???cell uptake and cytotoxicity were studied.The results show that the DAHnanoparticles with the core-shell structures have a high drug loading capacity???a good cycle stability and excellentphotothermal conversion efficiency under the irradiation of laser source.In phosphate buffer solution with a pH of7.4???DAH has high stability???the DOX leakage ratio in20h is less than20%???and DAH exhibits good stimulusresponse and can release the loaded drug quickly in acidic???hyaluronidase and photothermal action environment.In addition???DAH is more easily uptaken by tumor cells in cell experiments???which demonstrates certain targeting???showing better stimulus responsiveness.When chemotherapy and photothermal therapy works together???the activity oftumor cells is greatly weakened???showing the advantages and potential of combined therapy.