Diagnostic value of a sensitive next generation sequencing panel with unique molecular identifiers (umis) for evaluating routine eus-fna smears of solid pancreatic lesions

Aims Detection of clonal mutations may be of additional value for EUS-guided FNA. The amount of tissue on smears is often limited. Standard targeted next generation sequencing (NGS) approaches lack sensitivity to detect mutations in little material. The aim of this study was to investigate the diagnostic value of a NGS panel with unique molecular identifiers (UMI-NGS) on smears from solid pancreatic lesions with various cytological gradings according to the Bethesda criteria.