TiO2@Ag nanozyme enhanced electrochemiluminescent biosensor coupled with DNA nanoframework-carried emitters and enzyme-assisted target recycling amplification for ultrasensitive detection of microRNA
CHEMICAL ENGINEERING JOURNAL(2022)
摘要
MicroRNAs (miRNAs) have been discovered as promising biomarkers for cancer early diagnosis. Constructing sensitive biosensing strategy for detecting extremely low-abundance miRNAs in biological samples is urgently required. Herein, an efficient electrochemiluminescent (ECL) biosensor was developed for ultrasensitive detec-tion of miRNA coupled with DNA nanoframework-carried emitters (DNF-Dox-ABEI) and T7 exonuclease-assisted target recycling amplification (T7 exo-TRA). The fabricated DNF-Dox-ABEI emitters could load numerous luminophore molecules, and silver nanoparticles-coated TiO2 (TiO2@Ag NPs) nanozymes held excellent peroxidase-like activity in catalyzing H2O2 to produce enormous reactive oxygen species (ROS), which further reacted with ABEI to enhance ECL emission. Meanwhile, the T7 exo-TRA could effectively convert target hy-bridization event into amplifying signals for further improving sensitivity. Consequently, the constructed ECL biosensor enabled ultrasensitive detection of miRNA-155 with a broad linear range from 1.0 fM to 500.0 pM and a low detection limit of 0.45 fM (S/N = 3). Furthermore, it was noted that the biosensing strategy successfully achieved miRNA-155 analysis from breast cancer cells. Therefore, the constructed biosensor in the present study provided a potential tool in cancer early diagnosis and a novel approach for designing efficient ECL nanomaterials.
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关键词
MicroRNA analysis,DNA nanoframework,Nanozymes,Target recycling amplification,Electrochemiluminescent biosensor
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