Accelerated Neutral Atom Beam (ANAB) Modified Polypropylene for Reducing Bacteria Colonization Without Antibiotics.

For this first time, this study utilized Accelerated Neutral Atom Beam (ANAB) technology to modify polypropylene to inhibit bacteria colonization in vitro after 24 hours without the use of drugs or antibiotics. Specifically, ANAB was designed and used to increase the surface energy of polypropylene to be closer to that of two critical proteins (mucin and casein) contained in bodily fluids that if adsorbed to a material surface can decreased bacteria colonization. Materials were characterized using atomic force microscopy demonstrating an expected greater surface roughness and surface area for the ANAB-treated samples compared to controls. A wide range of gram-positive, gram-negative, and antibiotic resistant bacteria were tested here (including Staph. epidermidis, Staph. aureus, MRSA, multi-drug resistant E. coli, and Pseudomonas aeruginosa) and demonstrated on average an over a 3-log reduction in bacteria after 24 hours. Further, this study confirmed a greater adsorption of mucin and casein on ANAB-treated polypropylene as the mechanism to decrease bacteria colonization. Lastly, this study utilized an aggressive cleaning procedure and showed strong durability of the ABAN-treated surfaces. This study is important as it demonstrates a way to potentially decrease polypropylene based implant infections using ANAB modification without using antibiotics.