Coating Silica Layer on Fe3O4 Magnetic Nanoparticles and Application in Extracting High Quality Nucleic Acids from Blood Sample

The given research revealed that the size of Fe3O4 magnetic nanoparticles (MNPs) could be controlled by varying the pre-mixing conditions in the solvothermal method. Scanning electron microscopy (SEM) showed that the size of the MNPs gradually increased with increasing the initial temperature at which reaction components were mixed while the reaction component's mixing time was kept constant. The smallest sized MNPs were achieved among the five treatments (25, 50, 75, 100, and 125 ???C) when reactionIP: components14.98.160.66 wereOn: mixWed, at22 5 ???JunC, 2022while the06:31:08larger sized MNPs were synthesized among the five treatments when reaction components were mixed at 125 ???C. Then, Stober method was followed for coating Copyright: American Sc entific Publishers silica layer onto the MNPs. However, ammonium hydroxidwas replaced with potassium hydroxide as a catalyst, which Delivered by ngenta significantly increased the speed of silica coating onto MNPs. The Fourier transform infrared (FTIR) spectrometer revealed that the MNPs were successfully covered with silica in five minutes. FTIR spectra exhibited a peak about 1088.8 cm-1, which belonged to the asymmetry stretching vibration of Si-O-Si. Transmission electronic microscopy (TEM) analysis was conducted to confirm the presence of silica layer onto MNPs. Thus, potassium hydroxide was successfully employed as a catalyst for quick silica layer coating onto MNPs. Furthermore, these silica coated MNPs were used to extract high quality nucleic acids from blood sample.