SATB1 ensures appropriate transcriptional programs within naive CD8(+) T cells

Special AT-binding protein 1 (SATB1) is a chromatin-binding protein that has been shown to be a key regulator of T-cell development and CD4(+) T-cell fate decisions and function. The underlying function for SATB1 in peripheral CD8(+) T-cell differentiation processes is largely unknown. To address this, we examined SATB1-binding patterns in naive and effector CD8(+) T cells demonstrating that SATB1 binds to noncoding regulatory elements linked to T-cell lineage-specific gene programs, particularly in naive CD8(+) T cells. We then assessed SATB1 function using N-ethyl-N-nitrosourea-mutant mice that exhibit a point mutation in the SATB1 DNA-binding domain (termed Satb1(m1Anu/m1Anu)). Satb1(m1Anu/m1Anu) mice exhibit diminished SATB1-binding, naive, Satb1(m1Anu/m1Anu) CD8(+) T cells exhibiting transcriptional and phenotypic characteristics reminiscent of effector T cells. Upon activation, the transcriptional signatures of Satb1(m1Anu/m1Anu) and wild-type effector CD8(+) T cells converged. While there were no overt differences, primary respiratory infection of Satb1(m1Anu/m1Anu) mice with influenza A virus (IAV) resulted in a decreased proportion and number of IAV-specific CD8(+) effector T cells recruited to the infected lung when compared with wild-type mice. Together, these data suggest that SATB1 has a major role in an appropriate transcriptional state within naive CD8(+) T cells and ensures appropriate CD8(+) T-cell effector gene expression upon activation.