Temperature Effects on Plasma Li-heparin and Transaminase Activity in Children Blood with Tetralogy of Fallot

Abstract Tetralogy of Fallot (TOF) is a congenital heart disease that is caused by four heart defects, namely VSD, overriding aorta, obstruction of the right ventricular outflow tract and right ventricle hypertrophy. These abnormalities, which affect the anatomic structure of the heart, cause poor oxygenated blood to be pumped out of the heart. Children have smaller veins than adults, which make obtaining blood sample much harder. An experimental design was utilized, which allows the researchers to manipulate one or more variables and measure the resultant effects on the dependent variables. The population in this study was all vein blood sample of paediatric patients under the age of ten in National Cardiovascular Centre Harapan Kita Jakarta with TOF. Eleven sample were choose randomly as the sample in this study. We took 3 millilitres of blood from each sample and gave Li-heparin. The samples were then stored for 30 minutes at 5° C, 22-25° C and 37 ° C. We then used these sample to conduct ALT and AST tests. Based on the results of these tests we conducted descriptive statistical analysis, the normality test, homogeneity test and ANOVA test. We found that there is no significant difference in the results of AST and ALT tests using the three different samples. This study aimed to examine the effects of different temperatures on the volume of Li-heparin plasma and transaminase activity in human blood with Tetralogy of Fallot. The highest plasma volume was obtained from the sample that was put 30 minutes at 37°C.