Anaerobic Digestion of Corn Stover for Improved Biomethane Yield: Effect of Organic Nitrogen Sources (Soybean Curd Residue and Fish Waste)

The present study investigated the effect of organic nitrogen sources, soybean curd residue (SCR), and fish meal (FW) on the anaerobic digestion of corn stover for biomethane production. The bioreactors were seeded with the corn stover (corn cob and corn sheath), soybean curd residue (SCR), and fish waste (FW) at different combinations: (CC/SCR), (CC/FW), (CS/SCR) and (CS/FW), including CC and CS alone. The fermentation was for 31 days under mesophilic conditions. Characteristics of the substrates indicate that CC and CS are good carbon and energy sources, but low in nitrogen content. Conversely, SCR and FW are rich nitrogen sources, with low organic carbon content. There was a remarkable increase in biogas production in all treatments, except CC/SCR 75:25 and CC/SCR 85:15 in which inhibitory effect was observed. The highest percentage increase (138%) in biogas was recorded in CS/SCR 85:15 (2.86 dm3), and the least was CC/FW 75:25 with 1.49 dm3 (24.18% increase). Significant difference (P ≤ 0.05) in biogas yield was in the following: CC/SCR 50:50, CC/FW 50:50, CS/SCR 85:15, CS/FW 50:50, and CS/FW 75:25. The composition of the biogas revealed that the treatment improved biogas production as well as biomethane content, the highest being 69.44% in CS/SCR 85:15. Regression analysis of cumulative biogas yield as a function of time (t) in the different treatments that had a significant difference in biogas yield showed a good correlation between biogas yield (GY) and time (t). Improving the biodegradability of lignocellulosic wastes could lead to a boost in the development of anaerobic digestion and biogas production technology. To improve their biodegradability during anaerobic digestion, both pre-treatments and supplementation have vital roles to play.