Cycloartenol synthase in the amino acid residues and its contribution to phytosterol composition

The current work reports the role of cycloartenol synthase Rhizophora stylosa (RsCAS) and Kandelia candel (KcCAS) in the amino acid residue and the contribution from cycloartenol to the phytosterol synthase. Observation of the amino acid sequence highlights the significance of active sites of plant cycloartenol synthases using the CLUSTALW software. The phytosterol profile in the roots and leaves of R. stylosa and K. candel were analyzed using GC-FID and GC-MS. The residues of amino acid indicated for Tyr410 focused on the cycloartenol synthase in KcCAS and RsCAS, reinforcing the catalytic significance of this residue. The phytosterol pattern showed that 13-sitosterol is the main phytosterol composition both plants either in leaves or roots. This work found small content from cycloartenol of the leaves in K. Candel and R. stylosa, supporting those genes are involving for the phytosterol biosynthesis in these tree species.