Development and Validation of RP-HPLC Method for estimation of Secnidazole in API and Pharmaceutical Dosage Form

Objective of the present work is to develop and validate a simple, cost effective, sensitive and fast HPLC method for the analysis of Secnidazole. A Merck-Hitachi HPLC system with Peerless Basic C18 (50mm x 4.6mm x 3μm) column is employed for the analysis using buffer: methanol (80:20, v/v) as mobile phase. Signal from Secnidazole is detected at 310nm by UV Spectrophotometer. The proposed method is fully validated and found to be linear over a workable drug concentration, accurate, precise and robust. This fast and inexpensive method is suitable for research laboratories as well as for quality control analysis in pharmaceutical industries.