Abstract 322: Protein expression shift and potential diagnostic markers through proteomics profiling of A549 lung cancer cells

Abstract Highlights:-Adenocarcinoma lung cancer is common but difficult to diagnose. - Little is known for its proteome when methanol extract of Calotropis procera was used for the treatment groups. -In the treatment versus control groups, about 413 proteins were upregulated while about 626 proteins were downregulated. -Some of the proteins are potential diagnostic markers. Objectives: Adenocarcinoma lung cancer is a common type of lung cancer but the therapeutic and diagnosis are problematic. We focused on the protein expression of Adenocarcinoma lung cancer using A549 cancer cells and unveiled novel diagnostic makers and mechanisms in which methanol extracts of Calotropis procera ameliorate the disease. Methods: A549 lung cancer cell lines were obtained and we have 3 control groups and 3 treatment groups. The treatment groups were treated with 80 ug/ml of methanol extracts of Calotropis procera leaf and proteins were extracted for tandem mass tag mass spectrometry. Differential protein expression was performed between treatment groups and controls and the identified proteins were analyzed for pathway enrichment. Selected proteins were further validated in another set of samples using a more quantitative method. Results: In the treatment versus control, about 413 proteins were upregulated while about 626 proteins were downregulated. Conclusion: This balanced proteomics method not only offers important understandings of protein expression and pathways but also discover potential diagnostic markers for adenocarcinoma lung cancer. It also reveals the molecular mechanisms in which the methanol extracts of Calotropis procera leaf prevent carcinogenesis. Citation Format: Ayobami Matthew Olajuyin, Adefunke Kafayat Olajuyin, Ziqi Wang, Zhiwei Xu, Hayatu M. Raji, Malachy I. Okeke, Xiaoju Zhang. Protein expression shift and potential diagnostic markers through proteomics profiling of A549 lung cancer cells [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2021; 2021 Apr 10-15 and May 17-21. Philadelphia (PA): AACR; Cancer Res 2021;81(13_Suppl):Abstract nr 322.