Sensitive Electrochemical Detection of Nucleic Acids Following Electrokinetic Enrichment in a Bed of Bioconjugated Beads for Point-of-Care Testing
ECS Meeting Abstracts(2021)
摘要
In this presentation, we demonstrate that ion concentration polarization (ICP) in the presence of fluid flow drives focusing and efficient capture of target nucleic acids within a bed of oligoprobe-conjugated beads. A key finding is that ion conduction along the surface of the bioconjugated beads is the dominant contributor to current to the electrode that drives ICP, under the condition of ICP-induced depletion of ions from the bulk solution. Therefore, hybridization of a target nucleic acid (a polyanion) to the bead surface leads to a shift in the slope of the current-voltage curve. This approach is versatile in that a target nucleic acid can be detected electrically, in the absence of a label, or electrochemically via a label that generates an electroactive product. The resulting approach allows for a plug-and-play nucleic acid sensor using off-the-shelf bioconjugated beads and simple electronics, making it advantageous for point-of-care testing.
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