Identification of causal disease agent(s) of cherry bacterial canker in marmara region of turkey

Bacterial cancer diseases of cherry (Prunus avium L) caused by Pseudomonas syringae (P. syringae) is one of significant diseases in fruit producing areas of the World and can cause major economics losses. So, the survey study was conducted to obtain the P. syringae pathovar(s) in Marmara Region of Turkey. Tress with symptoms of bacterial canker were found in six cities of nine cities visited and ninety-five of hundred-seven orchards between 2016 to 2018 years in Marmara Region. Gram negative bacterial isolates were isolated from margin of necrotic tissue. All isolates were based on pathogenicity, LOPAT, and GATTa tests, 63 isolates were identified as P. s. pv. syringae (Pss). For molecular study, showed that all isolates produced on approximately 752 bp DNA fragment in PCR performed with primer set syrB1-syrB2. However, the results of MALDI- TOF MS analysis, the isolates were identified as Pss. According to the obtained results, it was concluded that Pss cause necrosis of cherry trees and its prevalence rate was determined as 58.88% in Marmara Region of Turkey.