Combined Oxidization and Liquid Ammonia Pretreatment of Bamboo for Maximizing Fermentable Sugars Release: Effects of Oxidant, Bamboo Age and Species

In order to figure out the potential effect for improving enzymolysis of biomass, the combined oxidization and liquid ammonia pretreatment (OD-LAT) was employed to bamboo. The effects of oxidant, bamboo age and species on pretreatment and following enzymolysis were studied. After optimal OD-LAT pretreatment and enzymolysis of B-NA, the glucan and xylan conversion reached 83.85% and 78.66% respectively, and about 59.7-68.5 g fermentable sugars could be produced per 100 g dry bamboo, which was roughly 7-9 fold comparing with untreated sample. The H 2 O 2 loading of 1.0 was the optimal oxidant dosage for the OD-LAT process. The OD-LAT pretreatment was only suitable for bamboo under 3-year-old, an it significantly improved the enzymolysis of B-NA and B-BM, while it was limited to B-DO and B-PP. The pretreatment and enzymatic hydrolysis effects of bamboo were not only related to composition, but also related to bamboo age, species, macro- and micro-structures.