Trapping and structural characterisation of a covalent intermediate in vitamin B₆ biosynthesis catalysed by the Pdx1 PLP synthase

Matthew J. Rodrigues,Nitai Giri,Antoine Royant,Yang Zhang, Rachel Bolton,Gwyndaf Evans,Steve E. Ealick,Tadhg Begley,Ivo Tews

RSC Chemical Biology（2022）

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摘要

Explaining stereoselective deprotonation and phosphate elimination in PLP biosynthesis through crystal structure, UV-vis absorption spectroscopic and mass spectrometric characterisation of a chromophoric intermediate.

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Trapping and structural characterisation of a covalent intermediate in vitamin B6 biosynthesis catalysed by the Pdx1 PLP synthase

Trapping and structural characterisation of a covalent intermediate in vitamin B₆ biosynthesis catalysed by the Pdx1 PLP synthase