Prospects for using pseudomonas zhaodongensis to mitigate herbicidal stress in wheat

The proliferation of herbicide-resistant forms of weeds provokes herbicide application in higher doses. It may have a negative impact on agricultural crops, causing oxidative stress, inhibiting the growth of plants, reducing yield potential. An important task is to find methods to mitigate herbicidal stress in crops. One approach may be to treat crops with microorganisms that favorably affect the growth of plants. Under the conditions of the light site, two-week wheat plants were sprayed with herbicides Octapon estra (0.1 µl/plant) based on 2,4-D and Nanomet (1.3 µg/plant) based on metsulfuron-methyl and a culture of bacteria 12N1 (107 CFU/plant). Herbicide-resistant strain 12N1, previously isolated from soil from the territory of a chemical industry enterprise (Republic of Bashkortostan, Russia), showed nitrogenase activity of 10.1 nmol C2H4•h-1•ml-1.The use of bacteria stimulated the growth of wheat roots both in the variants of the experiment with and without herbicides. Treatment with bacterial culture reduced the proline content in wheat leaves by 1.9 times against the background of the herbicide Octapon extra and by 6.6 times against the background of Nanomet, as well as the return of the total chlorophyll content to the control values. On the basis of the obtained data, the bacterial strain 12N1 was recognized as a potential antidote for mitigating herbicidal stress in wheat and was identified as member of the species Pseudomonas zhaodongensis based on the cultural, morphological, physiological, biochemical features and the sequence of the 16S RNA gene.