Study of the properties of the ovarian fluid of the Russian sturgeon (Acipenser gueldenstaedtii Brandt, 1833) during freezing

IOP Conference Series: Earth and Environmental Science(2021)

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摘要
Abstract The process of freezing the ovarian fluid of the Russian sturgeon (Acipenser gueldenstaedtii Brandt, 1833) was analyzed. With gradual cooling from 22°C to -196°C at a rate of 10° per minute, the process of freezing, formation and change of ice microparticles was observed. The ovarian liquid was glazed at -5°C and only at -70°. With the microparticles of ice began to form. From that moment, up to the temperature of liquid nitrogen (-196°C), the microparticles were crushed into smaller ones. The high content of intracellular water in the ovarian fluid of female Russian sturgeon contributes to the formation of the bulk of microparticles of ice, which is confirmed by their shape. These particles, which have sharp edges, are able to damage the thin shells of eggs during deep freezing.
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russian sturgeon,ovarian fluid
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