Characterization in terms of composition and phytotoxicity of aqueous spores extract

In this study, we used aqueous extracts of fern spores and solution of AgNO3 and HAuCl4 for the synthesis of bimetallic nanoparticles Au:Ag in different proportions: 1:1 and 1:10. The spores used come from 2 species of ferns: Asplenium scolopendrium and Dryopteris filix-mas. For the characterization of the extracts with or without bimetallic nanoparticles we applied Fourier transform infrared spectroscopy (FT-IR). Phytotoxicity was tested using Pisum sativum seeds. Each extract was tested in 2 dilutions: 1:10 (D10) and 1:100 (D100). The parameters, which we determinate were the root and stem growth and fresh biomass. Root growth was stimulated in variants with Asplenium scolopendrium extract: without nanoparticles both dilution and with Au:Ag nanoparticles 1:10 D10. The highest values obtained for the stem were at D10 at the variants with Asplenium scolopendrium extract with or without bimetallic nanoparticles. The influence of extracts on fresh biomass was smaller than on the growth of root and stem.