In vivo analysis of Ca<sup>2+</sup> activities in β-cells using a transgenic mouse line expressing a ratiometric Ca<sup>2+</sup> sensor
Proceedings for Annual Meeting of The Japanese Pharmacological Society(2022)
Abstract
Pancreatic β-cells release insulin to control glycemia in an intracellular Ca2+-dependent manner. Spatiotemporal dynamics and the function of Ca2+ signaling in β-cells have been studied by experiments using in vitro and ex vivo preparations. However, in vivo study remains challenging. Unlike the case of in vitro/ex vivo preparations, β-cells in vivo are under the influence of the autonomic nervous system, hormones, and other bioactive substances. Thus, β-cell Ca2+ activities under physiological conditions have not been clarified. We here report a method to analyze in vivo β-cell Ca2+ signals using a transgenic mouse line expressing a ratiometric Ca2+ indicator protein, YC-Nano50. Using the method, we visualized β-cell Ca2+ signals in laparotomized mice under anesthesia, and observed synchronized Ca2+ oscillations in β-cells within individual islets. Furthermore, we succeeded in monitoring Ca2+ activities in multiple islets simultaneously, which may provide unprecedented clues to understand the regulation mechanism of intravital insulin dynamics. Further studies in living animals using the new method are expected to help elucidate the mechanism of insulin secretion and the etiology of diabetes.
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Key words
transgenic mouse line,vivo analysis
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