UROTYPES IN YOUNG WOMEN WITH PELVIC PAIN: A PILOT ANALYSIS OF CLINICAL URINARY MICROBIOME CLASSIFICATION

You have accessJournal of UrologyCME1 May 2022MP49-20 UROTYPES IN YOUNG WOMEN WITH PELVIC PAIN: A PILOT ANALYSIS OF CLINICAL URINARY MICROBIOME CLASSIFICATION James M. Weinberger, L. Marcella Henao, Crystal Cisneros, James E. Ackerman, and A. Lenore Ackerman James M. WeinbergerJames M. Weinberger More articles by this author , L. Marcella HenaoL. Marcella Henao More articles by this author , Crystal CisnerosCrystal Cisneros More articles by this author , James E. AckermanJames E. Ackerman More articles by this author , and A. Lenore AckermanA. Lenore Ackerman More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000002624.20AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: There are substantial differences in the urobiomes of women depending on age and menopausal status. Our previous work demonstrated that younger women (age <45) tended to have a more limited set of dominant microbes and could be classified by Next Generation Sequencing into one of 3 “urotypes” based on the composition of Lactobacilli and E. coli. We sought to determine if we could confirm that pattern in an unselected sampling of women seeking urogynecologic care and if these patterns correlated with presenting complaints. METHODS: Women (age 18-45) presenting with LUTS were prospectively enrolled if their clean-catch urine sample was deemed unnecessary for analysis by the provider. Samples were assessed via quantitative PCR (qPCR) for E. coli and Lactobacillus crispatus, gasseri, iners and jensenii and classified into urotypes using unsupervised clustering. The subset of women complaining of painful urogenital conditions were classified into the following categories using ICD-10 codes and primary provider-given diagnosis: bladder-specific pain syndrome (BPS), myofascial pelvic pain (MPP), and unspecified pelvic pain (PP). We performed correlations between bacterial types and pain diagnoses. RESULTS: Using Lactobacillus spp. and E. coli, premenopausal women could be classified into 3 dominant urotypes; the majority of asymptomatic subjects fell into Urotype 1(low Lactobacilli, low E. coli). Of the 186 premenopausal women enrolled, 56 were diagnosed with BPS, MPP, or PP. Examining across urotype, the MPP and BPS groups segregate; patients with MPP were Urotype 1, while BPS exhibited elevated L. iners (Urotype 2) (Figure 1). The majority of PP patients were Lactobacillus-poor, falling into the E.coli-positive urotype (Urotype 3). CONCLUSIONS: Pelvic pain is a common complaint in this age group. While previous studies of pelvic pain have noted differences in diversity and Lactobacilli abundance between controls and pain groups, our qPCR-based urotyping was able to classify young women into three subgroups, demonstrating differential associations with asymptomatic controls and pelvic pain phenotypes. These data suggest that urotype data may be a meaningful mechanism to subclassify pelvic pain patients. Future studies are needed to determine if these groups correlate to treatment responses. Source of Funding: N/a © 2022 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 207Issue Supplement 5May 2022Page: e862 Advertisement Copyright & Permissions© 2022 by American Urological Association Education and Research, Inc.MetricsAuthor Information James M. Weinberger More articles by this author L. Marcella Henao More articles by this author Crystal Cisneros More articles by this author James E. Ackerman More articles by this author A. Lenore Ackerman More articles by this author Expand All Advertisement PDF DownloadLoading ...