Pd20-07 elevated ros levels and dna fragmentation in sperm from convalescent men in sars-cov-2 infection

You have accessJournal of UrologyCME1 May 2022PD20-07 ELEVATED ROS LEVELS AND DNA FRAGMENTATION IN SPERM FROM CONVALESCENT MEN IN SARS-COV-2 INFECTION Jorge Hallak, Felipe Bernardes, Thiago Afonso Carvalho Celestino Teixeira, Paulo Hilario Nascimento Saldiva, Esper Georges Kallas, Elia Tamaso Espin Garcia Caldini, Amaro Nunes Duarte Neto, Heloisa Faquineti, Vinicius Luiz Menezes Jesus, Raul Segundo Sanchez Gutierrez, and Joel R. Drevet Jorge HallakJorge Hallak More articles by this author , Felipe BernardesFelipe Bernardes More articles by this author , Thiago Afonso Carvalho Celestino TeixeiraThiago Afonso Carvalho Celestino Teixeira More articles by this author , Paulo Hilario Nascimento SaldivaPaulo Hilario Nascimento Saldiva More articles by this author , Esper Georges KallasEsper Georges Kallas More articles by this author , Elia Tamaso Espin Garcia CaldiniElia Tamaso Espin Garcia Caldini More articles by this author , Amaro Nunes Duarte NetoAmaro Nunes Duarte Neto More articles by this author , Heloisa FaquinetiHeloisa Faquineti More articles by this author , Vinicius Luiz Menezes JesusVinicius Luiz Menezes Jesus More articles by this author , Raul Segundo Sanchez GutierrezRaul Segundo Sanchez Gutierrez More articles by this author , and Joel R. DrevetJoel R. Drevet More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000002558.07AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Current evidence has proven the systemic nature of COVID19, including its involvement in the male reproductive tract. We aimed to investigate seminal parameters of moderate-to-severe COVID-19 men during the convalescence phase. METHODS: This cross-sectional study included 18 to 50-year-old men with confirmed moderate-to-severe COVID-19. Patients were enrolled 15 to 45 days after the diagnosis. After a urologist's initial clinical evaluation, semen samples were obtained by masturbation and processed within one hour. Semen analysis was performed using the World Health Organization (WHO) manual (6th edition). Sperm function tests were conducted in an andrology laboratory, including Reactive oxygen species (ROS), DNA fragmentation, lipid peroxidation, and Creatine Kinase (CK) analysis. An essential endocrine evaluation was performed. Patients with a history of disorders that could impair testicular function were excluded. A group of pre-vasectomy baseline samples was used as a control group. Statistical analysis was performed using R version 4.0.5. One-tailed and paired T-tests were used for comparisons between groups. RESULTS: The sample size was 26 men (mean 34.3±6.5 years; range: 21–50 years). Sperm concentration (mean 38.74±32, P <0.01) and total motile count (mean 55.3±66.8, P <0.01) were significantly reduced in the COVID-19 group. The DNA fragmentation (mean 41.1±29.2) and ROS (mean 4.84±8.7) were significantly higher in post-infection patients. Other parameters such as WHO/Kruger morphology and progressive motility were also reduced in the disease group, albeit not statistically significant. Total testosterone (mean 409.2±201.2) was lower in the convalescent men. All semen samples were negative for SARS-CoV-2 using the PCR analysis. CONCLUSIONS: Our findings indicate that male reproductive injury can be a relevant component of SARS-CoV-2 systemic infection. High DNA fragmentation and ROS, hallmarks of tissue injury, might signal a direct testicular involvement. The morphological and functional damage could represent significant impairment of the male reproductive health if persistent after convalescence. Source of Funding: None © 2022 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 207Issue Supplement 5May 2022Page: e358 Advertisement Copyright & Permissions© 2022 by American Urological Association Education and Research, Inc.MetricsAuthor Information Jorge Hallak More articles by this author Felipe Bernardes More articles by this author Thiago Afonso Carvalho Celestino Teixeira More articles by this author Paulo Hilario Nascimento Saldiva More articles by this author Esper Georges Kallas More articles by this author Elia Tamaso Espin Garcia Caldini More articles by this author Amaro Nunes Duarte Neto More articles by this author Heloisa Faquineti More articles by this author Vinicius Luiz Menezes Jesus More articles by this author Raul Segundo Sanchez Gutierrez More articles by this author Joel R. Drevet More articles by this author Expand All Advertisement PDF DownloadLoading ...