Effect of Potassium Dihydrogen Orthophosphate and Sucrose Concentration on Potato Growth and Development by In Vitro

Abstract Potato (Solanum tuberosum L.) is a staple food belonging to the three most important crops worldwide. In the recent five years, potato production in Indonesia tended to decline. One of the factors that cause the low productivity of potatoes is the limited availability of superior seeds. Micropropagation is the alternative to the conventional propagation of potatoes. The seed production technique of potatoes can be designed with in vitro multiplication through either plantlet regeneration or micro tuber production. This research aimed to investigate the effect of sucrose and potassium dihydrogen orthophosphate concentration on potato var Granola growth and development by in vitro. This research was conducted at the Plant Tissue Culture Laboratory, Proliteknik Negeri Lampung, from August to October 2021. Treatments were factorially arranged in a completely randomized design with three replications, each replication consisted of three culture bottles containing one explant. Data analysis using ANOVA and HSD test. The result of this research showed that the best growth and development of potato culture was obtained on media containing 340 mg.l-1 potassium dihydrogen orthophosphate and 60 g.l-1 sucrose.