The Identification 5S nrDNA Unit Classes in Genera of Plants and Selected Non-Vertebrate Animals and Their Potential for the Study of Species Relationships

OBM Geriatrics(2022)

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摘要
We have investigated the utility of the 5S nrDNA, a conserved, multicopy gene family organized into tandem repeats as a tool for genetic analyses in a wide variety of genera. Previous work in the Triticeae (Poaceae) demonstrated that the prior identification of unit classes based upon the 5S nrDNA NTS, greatly facilitates analysis. We investigated the potential of defining unit classes in other plant genera and several animal genera as a step towards future phylogenetic analyses. Our results demonstrate that in in several plant and animal genera there are sufficient numbers of DNA accessions in GenBank™ to point to different unit classes within species and that in some genera several potential unit classes are found across species. These results justify both more in-depth sampling within species and more breadth in sampling within. Further investigation of additional DNA sequences will help to define true unit classes.
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