Synthesis, Characterization of Cerium Oxide Nanoparticles and Evaluation of DNA Binding Interactions

The study focuses on investigating the DNA binding ability of cerium oxide nanoparticle (CeO NPs) for various biomedical applications. CeO NPs were synthesized by solution combustion method and hydrothermal method. The CeO NPs were characterized by Scanning electron microscopic technique and powdered Xray diffraction technique (XRD). Binding kinetics of CT-DNA and CeO NPs was studied using UV visible spectroscopy. Hypochromic shift was observed with the gradual addition of CT-DNA to CeO NPs indicating that the synthesized CeO NPs bind to CT-DNA by partial or non-intercalative binding. Based on the binding constant for the interaction between the DNA and CeO NPs, higher binding affinity was observed for CeO NPs synthesized by combustion method compared to hydrothermal method. This shows that the method of synthesis has impact on the interactions between nanoparticles and CT-DNA.