miR-182通过RAC1通路促进骨肉瘤HOS细胞凋亡
Chinese Journal of Immunology(2021)
Abstract
目的:检测miR-182在骨肉瘤HOS细胞中的表达,通过构建过表达miR-182的转染载体检测miR-182对骨肉瘤HOS细胞凋亡的影响.方法:RT-qPCR检测骨肉瘤HOS细胞miR-182表达.构建过表达miR-182的转染载体,进行转染后细胞共培养,RT-qPCR检测转染后miR-182表达,显微镜下观察细胞形态.Western blot分析miR-182对RAC1通路蛋白的作用,CCK-8法分析过表达miR-182对骨肉瘤HOS细胞生长的抑制情况,流式细胞术评估miR-182对骨肉瘤HOS细胞细胞周期及凋亡的影响.结果:骨肉瘤HOS细胞中miR-182呈低表达.构建过表达miR-182的转染载体,骨肉瘤HOS细胞中RAC1通路蛋白及下游PAK1蛋白表达显著下调.过表达miR-182显著促进骨肉瘤细胞凋亡并阻滞其细胞周期进程.结论:miR-182可通过靶向调控RAC1通路促进骨肉瘤HOS细胞凋亡并阻止其细胞周期进程.
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