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等位基因特异性PCR法快速检测烟曲霉常见耐药突变基因

DENG Yuchen,FANG Wenjie,DENG Shuwen,CHEN Xianzhen, ZHANG Yaru,FENG Zhenyi,LING Liyan,PAN Weihua,LIAO Wanqing

Chinese Journal of Mycology（2021）

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Abstract
目的 建立一套检测烟曲霉L98H/TR34耐药突变的二重扩增体系,进而快速准确判断其对于唑类的耐药情况.方法 通过等位基因特异性PCR法实现L98H/TR34耐药突变二重PCR检测,评价该体系的特异性和敏感性.结果 所有烟曲霉L98H/TR34耐药基因阳性菌株实时荧光定量PCR(Real Time PCR,RT-PCR)溶解曲线均呈现双峰,无突变的菌株均未检测到突变位点.结论 等位基因特异性PCR能够快速检测烟曲霉菌常见耐药突变基因L98H/TR34,具有较高的特异性和敏感性,操作简单,价格低廉.
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