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HPLC法测定灯盏细辛中4种酚性成分的含量

Jiangsu Agricultural Sciences(2013)

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Abstract
建立了中药材灯盏细辛中1,3-二咖啡酰基奎宁酸、灯盏花乙素、3,4-二咖啡酰基奎宁酸、3,5-二咖啡酰基奎宁酸的高效液相色谱含量测定方法.采用C18(250 mm×4mm,5μm)分析柱;流动相为乙腈-0.2%甲酸溶液(5:80)(A)、四氢呋喃(B);流速0.800 mL/min;柱温30℃;检测波长335 nm.结果表明,上述4种成分分别在0.02044~0.8176 μg(r =0.999 1)、0.020 34~0.813 67 μg(r =0.999 8)、0.020 08~0.803 2μg(r=0.999 8)、0.020 72~0.828 8 μg(r =0.999 0)范围内线性关系良好,加样回收率分别为97.71%(RSD=1.4%)、99.32%(RSD=1.2%)、98.43%(RSD =2.3%)、98.62%(RSD =2.7%).本法简便快捷,结果准确,重复性好.
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