Mono-PEGylated lysozyme purification with increased productivity and isomer differentiation through heparin monolith chromatography

Luis Alberto Mejía-Manzano,Víctor R. Campos-García,Francisco C. Perdomo-Abúndez,Emilio Medina-Rivero,José González-Valdez

Journal of Chromatography B（2022）

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摘要

•An affinity monolith chromatography is proposed using a disk alkylated reductively with heparin.•20 kDa mono-PEG lysozyme purity was comparable with traditional column method.•20 kDa mono-PEG lysozyme productivity increased 6.1 times compared to packed-bed affinity.•Positional isomers of 20 kDa mono-PEGylated lysozyme were partially discriminated.

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关键词

Affinity monolith chromatography,Heparin,Mono-PEGylated lysozyme,Lysozyme PEGylation reaction,Reductive alkylation

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