Real-time imaging of structure and dynamics of transmembrane biomolecules by FRET-induced single-molecule fluorescence attenuation
Biophysics Reports(2021)
摘要
Tracking the transmembrane topology and conformational dynamics of membrane proteins is key to understand their functions.It is however challenging to monitor position changes of individual pro-teins in cell membranes with high sensitivity and high resolution.We review on three single-molecule fluorescence imaging methods—SIFA,LipoFRET and QueenFRET—recently developed in our lab for studying the dynamics of membrane proteins.They can be applied,progressively,to investigate mem-brane proteins in solid-supported lipid bilayers,artificial liposome membranes and live-cell plasma membranes.The techniques take advantage of the energy transfer from a fluorophore to a cloud of quenchers and are able to extract in real time positions and position changes of a single fluorophore-labeled protein in the direction normal to the membrane surface.The methods have sub-nanometer precision and have proved powerful to investigate biomolecules interacting with bio-membranes.
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关键词
Single-molecule fluorescence imaging,Dynamics of membrane proteins
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