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Hi-Ⅱ玉米的NGc抗虫基因遗传转化研究

LIU Yue,WANG Li-da,LAN Ying,LI Qing-chao,LIU Yang,ZHNEG Xu,ZHAO Xiu-mei

Heilongjiang Agricultural Sciences（2022）

Cited 1|Views22
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Abstract
为促进转基因抗虫玉米研究,通过农杆菌介导的玉米幼胚遗传转化法将目的基因转入玉米材料Hi-Ⅱ中,通过外植体侵染、共培养以及分化筛选,培养具有CryNGc抗虫基因的玉米植株,并研究外源基因在后代的遗传表达及抗性情况.结果表明:玉米幼胚大小在1.2～1.8mm时侵染效果最好,并成功获得了10株具有Cry NGc抗虫基因的阳性转化植株,转化效率为1.428‰.
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