UPLC-MS联用同时测定建泽泻中8种三萜类化学成分
Chinese Journal of Ethnomedicine and Ethnopharmacy(2022)
Abstract
目的:建立建泽泻中泽泻醇A等8种三萜类成分含量的UPLC-MS联用测定方法.方法:WatersCORTEC-SC1s色谱柱(2.1mm×100 mm,1.6 μm),流动相0.1%甲酸水-乙腈,流速0.25 mL/min,柱温40℃,进样量2μL,梯度洗脱;质谱条件采用电喷雾正离子模式,毛细管电压3.50kV,脱溶剂气流氮气800 L/h,脱溶剂温度500℃,锥孔气流氮气150 L/h,离子源温度150℃,二级锥孔萃取电压3.00V,碰撞气体氩气.结果:泽泻醇A等8种三萜类成分在0.01~10.0 mg/L范围内,待测成分峰面积/内标物质甘草次酸峰面积比值与浓度线性关系良好,泽泻中23乙酰泽泻醇B含量最高(0.5182~1.2836 mg/g),泽泻醇A含量最低(0.0076 ~0.0078 mg/g).结论:方法准确灵敏、简便可靠,可为建泽泻品质评价提供新的分析手段.
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