云南地方品种小黄姜快繁技术研究
Chinese Journal of Tropical Agriculture(2022)
Abstract
建立云南地方品种生姜快繁体系,为姜种的快速繁殖提供技术保障,同时为生产应用提供技术支撑.以云南文山地方品种小黄姜为实验材料,研究其组培快繁技术.结果表明:最佳消毒方式是75%酒精消毒20 s+2%次氯酸钠消毒15 min+无菌水清洗3次;最佳诱导培养基是MS+6-BA 1.0 mg/L+NAA 0.1 mg/L+琼脂7 g/L+蔗糖30 g/L,pH 5.8;最佳继代培养基MS+琼脂7 g/L+蔗糖30 g/L,扩繁系数达5;最佳生根培养基1/2 MS+NAA 0.1 mg/L+卡拉胶7 g/L+蔗糖30 g/L;最佳移栽基质是营养土:椰糠1:1(m:m),移栽成活率达95%以上.
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