补肾健骨胶囊中莫诺苷与马钱苷含量测定

Ming Kaili, Wang Haiding, Zhang Xinzhi, Tian Zhiyang,Huang Zhijun,Xiang Yang, Chen Peng,Zeng Qinghui

China Pharmacist（2022）

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Abstract

目的:建立一种同时检测补肾健骨胶囊中莫诺苷和马钱苷含量的方法.方法:采用Tnature C18(250 mm×4.6 mm,5μm)色谱柱,柱温30℃,以乙腈-0.8％磷酸溶液为流动相,梯度洗脱,体积流量1.0 ml?min-1,检测波长240 nm.结果:莫诺苷在82.0108～1640.2160 ng、马钱苷在63.7560～1275.1200 ng范围内线性关系良好;莫诺苷的平均回收率为94.38％,RSD为2.06％,马钱苷的平均回收率为94.90％,RSD为1.88％(n=6).结论:该方法操作简便,重复性好,可准确检测补肾健骨胶囊中莫诺苷和马钱苷的含量.

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