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siRNA抑制牛睾丸细胞Nrf2基因表达的研究

HU Xiu-zhong,XIANG Min,YU Jie,LIU Chen-hui,XIA Yu,TAO Bi-fei,ZHOU Yuan,WANG Ding-fa,CHENG Lei

China Dairy Cattle（2022）

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Abstract
为了探讨Nrf2基因被干扰后对牛睾丸细胞的影响,试验采用了实时荧光定量PCR和Western boltting方法检测了牛睾丸细胞中HO-1和NQO1基因的表达.结果显示,设计的siRNA-1672在终浓度为50nmol/L时抑制效果较好,Nrf2基因表达显著下降,HO-1和NQO1基因在mRNA水平上表达显著下降.Nrf2被干扰72h后,HO-1和NQO1蛋白质水平表达水平明显下降.说明Nrf2对牛睾丸细胞中HO-1和NQO1基因的表达存在调控作用.
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