UPLC-MS/MS法同时测定人血浆中泊沙康唑、氟康唑、伏立康唑的血药浓度
Journal of Tianjin Medical University(2022)
Abstract
目的:建立UPLC-MS/MS法同时测定血浆中3个三唑类抗真菌药物(泊沙康唑、氟康唑、伏立康唑)的浓度.方法:采用WatersBEHC18(2.1 mm×50 mm,1.7μm)色谱柱,流速0.5 mL/min,流动相为甲醇(含2 mmol/L乙酸铵和0.1%甲酸)和2 mmol/L乙酸铵水溶液(含0.1%甲酸),梯度洗脱,柱温40°C.质谱条件采用电喷雾离子源,正离子多反应监测模式进行扫描分析.结果:泊沙康唑、氟康唑、伏立康唑的专属性良好,线性范围分别为0.083~20.680、0.328~82.075、0.080~20.085μg/mL,范围内线性关系良好,r均大于0.99.批内、批间精密度(RSD)均小于7.60%,提取回收率和基质效应RSD值均小于14.2%,不同储存条件下稳定性良好,RSD值均小于10.69%.结论:该方法准确度高,重复性好,简便快捷,可同时测定3种血浆中三唑类抗真菌药的浓度,适用于临床对氟康唑、泊沙康唑及伏立康唑的治疗药物监测.
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