中药药敏试验新方法及68种中药对克氏原螯虾病原菌的抑菌活性研究
Journal of Anhui Agricultural Sciences(2022)
Abstract
为消除中药颜色和不溶颗粒对最小抑菌浓度测定结果判定的影响,建立了微量肉汤稀释联合平板计数法.结果发现,抗菌剂(磺胺甲噁唑、多西环素、氟苯尼考、硫酸新霉素、恩诺沙星)MIC测定前后的MIC孔细菌浓度比值(BCRMIC)为10-3~103.以BCRMIC为标准替代肉眼观察来判定中药MIC,采用微量肉汤稀释联合平板计数法定量评价了68种中药的抗菌活性.五倍子、黄连、山楂、地锦草、厚朴、黄芩、石榴皮、翻白草、乌梅对克氏原螯虾源病原菌(嗜水气单胞菌、维氏气单胞菌、弗氏柠檬酸杆菌)的抗菌活性较强,其MIC为4~16 mg/mL.
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