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蓝尾蝾螈皮肤转录组及活性多肽分析

Journal of Fujian Agriculture and Forestry University(Natural Science Edition)(2022)

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Abstract
通过对蓝尾蝾螈皮肤进行转录组测序,首次报道了蓝尾蝾螈皮肤的转录组及其活性多肽成分.采用Illumina Hiseq4000共测定了6.65 Gb数据;组装和去冗余后共获得75462个unigene,其N50、GC含量、平均长度、总长度分别为1617 bp、45.64%、818 bp、61801999 bp.通过基因注释,共获得34850个非冗余注释unigene(占unigene总数的46.18%),注释结果为两栖类物种的unigene数目约占注释总数的16%.根据unigene的COG和GO注释结果,统计了各自的功能分类.其中,对鉴定到的多种活性多肽进行了初步的生物信息学分析,为诠释蓝尾蝾螈皮肤生理学功能提供依据.
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