果桑离体冬芽组培快繁和枝条扦插快繁体系的建立
Molecular Plant Breeding(2021)
Abstract
本研究采用组织培养和扦插再生技术对四季果桑和鸡爪桑的冬芽和枝条进行无性繁殖.结果 表明:2个品种均可进行再生培养,其中四季果桑增殖培养的最佳培养基为MS+1.5 mg/L 6-BA+0.1 mg/L NAA,增殖系数为3.167,最适生根培养基为1/2MS+2.0 mg/L NAA,生根率达100%,平均主根数11.167,根长3.734 cm;鸡爪桑增殖培养的最佳培养基为MS+1.5 mg/L 6-BA+0.1 mg/L NAA,增殖系数为3.667,最适生根培养基为1/2MS+1.0 mg/L IBA,生根率65.5%,平均主根数6.5,根长3.081 cm;2个品种的移栽成活率均可达100%.扦插试验表明,草炭土∶壤土∶珍珠岩=2∶1∶1是2个果桑品种最佳扦插基质,成活率分别为75%和81.8%.
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