蛹虫草发酵产虫草素工艺优化

Food and Fermentation Industries(2022)

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Abstract
为确定蛹虫草产虫草素放大工艺条件,考察了菌种培养质量和发酵高径比对虫草素合成的影响.确定种子培养最佳条件为:转速250 r/min,接种量15%,扩培级数2次,此条件下虫草素发酵产量可达6g/L左右.另外,装液量最适高径比为2 cm/30 cm.在此条件下,进行了120 L多层反应器发酵工艺验证.发酵25 d,虫草素产量为5.32 g/L,转化率57%,与小试工艺结果基本一致.实验结果表明,工艺放大具有应用可行性,为进一步工业化生产提供了参考.
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