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'章姬'草莓茎尖脱毒及快繁技术研究

CHEN Ganniu,MIAO Jie,LU Zhaojun,ZHAO Ying,SUN Xiaohui,SONG Li,WANG Shiwei,QIAO Yanhui,CHAI Chuanping

Journal of Shandong Forestry Science and Technology（2022）

Cited 0|Views15
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Abstract
本研究以'章姬'草莓为试验材料,通过外植体消毒、高温处理剥离茎尖、愈伤组织诱导、不定芽诱导、生根培养建立'章姬'草莓茎尖脱毒及快繁体系.'章姬'草莓茎尖脱毒以38℃高温连续处理38 d为宜;最佳的灭菌处理为75％酒精60s,2％次氯酸钠8min;茎尖愈伤组织诱导培养基为MS+6-BA 1.5 mg·L-1+ IBA 0.75 mg·L-1;不定芽诱导培养基为MS+6-BA1.5 mg·L-1+NAA 0.5 mg·L-1,最佳生根培养基为1/2 MS+NAA 0.75 mg·L-1.建立的茎尖脱毒及快繁体系为'章姬'草莓的开发利用以及工厂化育苗提供参考.
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