Effect of Selenium Nanoparticles and Chitosan on Production Performance and Antioxidant Integrity of Heat-Stressed Broiler

In this study, 336-day-old corn cob broilers were bought for the poultry experimental station during the months of May and June 2021. Before the arrival of chicks, the brooders, chick feeders, drinkers, humidity, temperature, and feeding management were controlled according to scientific patterns. These birds were randomly divided into seven groups and six replications of eight birds, viz. Group-A (positive control on basal diet only), Group-B (negative control on basal diet and HS), group-C (basal diet + simple Se 0.3 mg/kg feed), Group-D (basal diet + SeNP 0.3 mg/kg feed + HS), Group-E (BD + HS + chitosan), Group-F (BD + Se + COS), and Group-G (nano Se with chitosan 0.3 mg/kg + BD + HS). On the 42 nd day of research, two birds were selected from each replication and sacrificed after blood collection. The initial data related to feeding intake, live body weight, and feed conversion ratio (FCR) were collected before slaughter. The intestinal samples were collected and immediately transferred to formalin after grass morphometry. The live body weight, FCR, feed intake, intestinal histomorphology, relative organ weight, and antioxidant parameters like MDA, SOD, and GPX were significant ( P > 0.005) in all groups, with Group-G at the highest, followed by Groups-F, E, D, C, A, and B. Group-B (negative control group) was the most affected group in all aspects because of heat stress and only basal diet. It was concluded that heat stress highly causes a loss in performance, intestinal gross morphology, and histology in poultry, and increases stress conditions, whereas the selenium nanoparticle works to improve the body weight, FCR, and intestinal parameters.