Immunolocalization of Notch Signaling in Mouse Preimplantation Embryos.

Elisabete Silva,Patrícia Diniz,Alexandre Trindade,Mariana Batista,Ana Torres,António Duarte,Luís Lopes-da-Costa

Methods in molecular biology (Clifton, N.J.)（2022）

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摘要

The Notch signaling pathway is an important determinant of cell diversity and identity in most developing embryonic tissues. The pathway components are expressed dynamically, and their function is critical for embryonic survival.This protocol addresses the immunolocalization of Notch pathway components by confocal microscopy.

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关键词

Confocal microscopy,Immunocytochemistry,Mouse,Notch intracellular domain (NICD),Notch signaling,Preimplantation embryo

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