Saccharopolyspora soli sp. nov., isolated from Northern Cyprus soil

A novel Gram-stain positive, aerobic, non-motile actinobacterium, designated strain K220 T , was isolated from soil collected from Cape Andreas (Zafer Burnu), Northern Cyprus, and subjected to a polyphasic taxonomic approach. The organism was shown to have phylogenetic, chemotaxonomic, cultural and morphological properties consistent with its classification in the genus Saccharopolyspora . 16S rRNA gene sequence analysis of strain K220 T showed that it is closely related to the type strains of Saccharopolyspora maritima 3SS5-12 T , Saccharopolyspora kobensis JCM 9109 T and Saccharopolyspora hirsuta ATCC 27875 T with 97.6, 97.5 and 97.0% sequence similarity, respectively. In silico DNA–DNA hybridization and average nucleotide identity values between strain K220 T and type strains of the genus Saccharopolyspora with publicly available genomes were 22.1–31.2% and 76.0–83.16%, respectively. The DNA G + C content of strain K220 T was 68.3 mol%. The genome of strain K220 T has genes associated with 24 biosynthetic gene clusters. The strain contained MK-9(H 4 ) and iso -C 16: 0 as the predominant respiratory quinone and fatty acid, respectively. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine. Based on evidence collected from the genotypic, phenotypic and phylogenetic analyses, strain K220 T is considered to represent a novel species in the genus Saccharopolyspora , for which the name Saccharopolyspora soli sp. nov. is proposed. The type strain is K220 T (= JCM 33912 T = KCTC 49395 T ).