Functional roles for AGPAT6 in milk fat synthesis of buffalo mammary epithelial cells.

AGPAT6 plays a crucial role in the triglyceride (TG) synthesis pathway in mammals. However, its roles in buffalo lactation remain unknown. Therefore, we investigated the functional roles of in milk fat synthesis by transfecting overexpression and lentivirus interference vectors in buffalo mammary epithelial cells (BuMECs) in vitro. overexpression in BuMECs significantly enhanced the mRNA expression of , , , , , , and genes, and significantly reduced that of , , , and , but has no significant influence to the mRNA abundance of , and . However, the interference with , the mRNA expression of , , , , , , , , , , and genes in BuMECs changed contrary to the overexpression experiment, and that of , and also did not change significantly, but the expression of was significantly decreased. In addition, the overexpression/interference of gene significantly increased/decreased TG content in BuMECs. The results here indicate that gene is involved in TG synthesis in BuMECs, and affects the expression of major genes associated with FA transport and activation, TG synthesis and transcription regulation, FA oxidation and TG degradation during the lipogenesis of milk.