Chrome Extension
WeChat Mini Program
Use on ChatGLM
VIPCreated with Sketch.

NDV基因Ⅶ型DNA纳米颗粒疫苗初探

WU Yaotang,LIANG Jianpeng, LIN Sixian,LIU Hongzhi,XIANG Bin,DING Chan,XU Chenggang,LIAO Ming,REN Tao

Chinese Journal of Veterinary Parasitology（2022）

Cited 1|Views4
No score
Abstract
本研究利用PCR方法扩增新城疫病毒(NDV)rGM株的F基因,并将其克隆至真核表达载体pCAGGS,构建重组表达质粒pCAGGS-F.采用离子交联法将壳聚糖、MgSO4、pCAGGS-F以及三聚磷酸钠(TPP)混合,制备成新城疫DNA纳米颗粒疫苗.IFA试验检测结果表明,F重组蛋白成功表达.制备的纳米颗粒疫苗通过点眼滴鼻免疫2周龄SPF鸡2次后,对103 EID50 NDV强毒株rGM保护率可达100％.此试验为新城疫DNA纳米疫苗等的研制奠定基础.
More
AI Read Science
Must-Reading Tree
Example
Generate MRT to find the research sequence of this paper
Chat Paper
Summary is being generated by the instructions you defined