NDV基因Ⅶ型DNA纳米颗粒疫苗初探
Chinese Journal of Veterinary Parasitology(2022)
Abstract
本研究利用PCR方法扩增新城疫病毒(NDV)rGM株的F基因,并将其克隆至真核表达载体pCAGGS,构建重组表达质粒pCAGGS-F.采用离子交联法将壳聚糖、MgSO4、pCAGGS-F以及三聚磷酸钠(TPP)混合,制备成新城疫DNA纳米颗粒疫苗.IFA试验检测结果表明,F重组蛋白成功表达.制备的纳米颗粒疫苗通过点眼滴鼻免疫2周龄SPF鸡2次后,对103 EID50 NDV强毒株rGM保护率可达100%.此试验为新城疫DNA纳米疫苗等的研制奠定基础.
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