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高效液相色谱-示差折光检测器检测鱼油甘油酯含量

China Food Additives(2021)

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Abstract
建立了高效液相色谱--示差折光检测器测定鱼油甘油酯含量的方法.选用Agilent ZORBAX RX-SIL色谱柱(4.0mm×250mm,5μm),正己烷和异丙醇为流动相(15:1,v:v),流速0.8mL/min,柱温34oC,示差折光检测器(RID)检测.结果表明,油酸甘油三酯和油酸甘油一酯在5.0~25.0mg/mL范围内,方法的线性关系良好,相关系数分别为0.9999和0.9998;加标回收率分别为97.35%~100.47%、98.32%~99.65%,相对标准偏差(RSD)分别为1.59%、0.68%.该方法操作简便、精密度和方法重复性好,适用于鱼油甘油酯含量测定.
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